LIPID PEROXIDATION AND NON-ENZYMATIC ANTIOXIDANTS STATUS IN PREECLAMPTIC AND POSTPARTUM PREECLAMPTIC WOMEN

ABSTRACT

Context: Preeclampsia is a hypertensive disorder and is one of the most leading cause for maternal and fetal mortality in developing countries.
Aim: Objective of this study was to investigate the lipid peroxidation and non-enzymatic antioxidants status in women with preeclampsia and compare the same parameters in women with normal pregnancy and postpartum preeclampsia women.
Settings and Design: District civil hospital, Case control study.
Methods and Material: The study comprised of 25 normal healthy non pregnant controls, 25 normal healthy  pregnant women in 3rd trimester and 25 preeclamptic  women in 3rd trimester. The same 3rd trimester preeclamptic women were used for follow up study after delivery and were considered as postpartum preeclamptic group. Whole blood was used to detect Malondialdehyde (MDA) a product of lipid peroxide, nonenzymatic antioxidants like reduced glutathione, vitamin E, vitamin-C and vitamin-A. Statistical significance was determined by ANOVA and multiple comparison tests.
Results: There was a consistent significant increase in lipid peroxidation (MDA) in all the groups as compared to nonpregnant controls (P< 0.001). Elevated levels of malondialdehyde in pre-eclamptic subjects declined significantly (p<0.001) after delivery. A significant
decrease (P<0.001) in the level of non-enzymatic antioxidants viz. reduced glutathione, vitamin-E, vitamin -A and vitamin – C was observed in all the groups as compared to nonpregnant controls. In the postpartum preeclamptic group significant increase was noted in all antioxidants except vitamin A as compared to preeclamptic group.
Conclusions: Our study showed clear insight into disturbances associated with normal pregnancy, which are exaggerated in  complicated pregnancy like preeclampsia with enhanced lipid peroxidation and decreased antioxidants. Early attention, intensive
management and treatment are essential to improve maternal and fetal outcome

Oxidative Stress And Enzymatic Antioxidants In Acute Myocardial Infarction

ABSTRACT
Myocardial infarction (MI) is the single largest killer disease in the world. Oxidative stress is one  of the hallmarks in patients with acute myocardial infarction as observed in recent years.The objective of the study was to evaluate oxidative stress and antioxidant enzyme activity in patients of acute MI.The present study comprised of 50 clinically diagnosed and ECG confirmed cases of acute MI and 50 healthy controls. Oxidative stress marker malondialdehyde (MDA) and enzymatic antioxidants like
superoxide dismutase (SOD), catalase and glutathione reductasewere estimated in the cases and controls.Statistical analysis  as carried out by using students’t’ test (unpaired). Correlation analysiswas done by using SPSS software version 16.The MDA level was significantly increased (P< 0.0001) in the cases (11.5 ± 0.72 nmol/ml) as compared to controls (6.3 ± 0.92 nmol/ml). The activity of SOD, glutathione reductase and catalasewere significantly low (P< 0.0001) in acuteMI (450.0 ± 116.76 IU/g Hb, 3.1 ± 0.79
IU/g of Hb and 2.6 ± 0.53 IU/g of Hb respectively) as compared to controls (940.4 ± 93.9 IU/g of Hb, 9 ± 1.02  IU/g of Hb and 7.3 ± 0.70 IU/g of Hb respectively). A significant negative correlation was detected between malondialdehyde and catalase activity in acute MI (r= – 0.339; p<0.02).The study indicates increased oxidative stress and decreased antioxidant defence in patients of acutemyocardial infarction. Antioxidant supplements are compounds obtained either by extraction from natural foods (dietary ntioxidants) or by chemical synthesis  (synthetic antioxidants). Many epidemiological data suggest that synthetic antioxidant supplementation may have a beneficial effect inmany chronic diseases. So novel t h e ra p e u t i c approaches l i ke ant i ox i d a nt supplementation may be useful in the management of persisting oxidative stress in acuteMI.