DENGUE: SEROPREVALENCE, COMPARISON OF RAPID TEST WITH ELISA

ABSTRACT

Introduction: – Dengue is a major health problem in many parts of the tropical world. It is a mosquito borne illness caused by one of the serotypes of dengue viruses.
Aims and objectives: – The present study was done to
know the common clinical features and Seroprevalence of dengue in our region. An attempt was made to compare rapid test SD dengue duo (IgM, IgG & NS-1 Ag detection) with capture ELISA (IgM, IgG Microlisa
Dengue).

Materials & Methods: – 226 serum samples were tested in patients clinically suspected Dengue. All the 226 samples were subjected to IgG, IgM Microlisa test. The same were put on rapid SD bioline Dengue duo rapid test and was compared with ELISA.
Results:- 226 serum samples were tested in patients clinically suspected Dengue before noting common clinical signs and symptoms. 150 samples were tested positive with ELISA (either positive for IgG, IgM or both). Seroprevalence of 66% were reported. When compared with ELISA, Rapid test showed sensitivity of 80.6% specificity and positive predictive value of 100% & zero false positive rates. Efficiency of the test was 87.16%
Conclusion:- High prevalence rate in our region particularly in premonsoon & monsoon season gives an alarm to the doctors regarding early and accurate diagnosis of dengue virus infection. SD Dengue duo rapid test should be a valuable screening test for dengue fever
which can be interpreted easily. Results were comparable to ELISA. It provides additional diagnostic investigation that compliments NS-1 antigen detection.

DENGUE & CHIKUNGUNYA VIRUS FEVER OUTBREAKS IN DELHI, IG-M SEROLOGY STATUS – A RECENT EXPERIENCE

ABSTRACT

Dengue and Chikungunya virus illness affects tropical and subtropical regions around the world including India. In Delhi,a highly populous city, as recently as in the year 2010, several cases of fever with clinical picture similar to that of dengue and chikungunya virus infection eported in our Vardhman Mahavir Medical College & associated Safdarjang Hospital, New Delhi. Cases of fever started reporting in late June, 2010 but fromSeptember onwards also emerged cases of chikungunya. On clinical basis alone, especially during mixed outbreaks, it is generally difficult to differentiate between the two, in particular when dengue fever does not manifest as DHF. Serology
for dengue and chikungunyawas performed byMacELISA test with the aim as detection of specific IgM antibodies. In cases of dengue fever from late June till end August – 2010, the IgM seropositivitywas found to be 38.3%. From September, 2010 onwards and till December end, in cases of chikungunya virus fever the IgM seropositivity was found to be 65.11% and that of dengue as 37.86%. Also, in cases from Sep to Dec, 2010, which presented with clinical pictures suggestive of both types of viral infections, revealed that the seropositivity for dengue declined to 8.36%, and for Chikungunya increased to 41.46%. In conclusion, the dengue outbreak, especially in city Delhi, may get overwhelmingly replaced by chikungunya virus fever cases with both the dominant viruses’ co circulating in the community. Detection of IgM antibodies against dengue and chikungunya by ELISA during the mixed outbreak appears to play important role for distinguishing the two, and reinforces clinical diagnosis, and, hence, helps initiate proper medical care.