LIPID PEROXIDATION IN ACUTE MYOCARDIAL INFARCTION BEFORE AND AFTER REPERFUSION

ABSTRACT

Coronary artery disease is caused by the additive and interactive effects of several factors. Substantial evidence indicates that Reactive Oxygen Species (ROS) play a vital role in the etiopathogenesis of atherosclerosis. Our study has been designed to evaluate the lipid peroxidation due to ROS in Acute myocardial infarction (AMI) and assess the antioxidant protection against ROS. The study comprised of 50 AMI patients and 50, age and sex matched healthy subjects. Lipid peroxidation was measured as Malondialdehyde (MDA) levels in serum which was observed to be significantly high in AMI patients in our study. The activity of Superoxide Dismutase (SOD) in serum was found significantly decreased in patients with AMI. The estimation of MDA and SOD in serum of patients who underwent thrombolysis for Reperfusion indicated a further rise in serum MDA level and a decrease in serum SOD level which were statistically significant. AMI patients who were habitual smokerswere also assessed for their serum MDA and SOD levels. After reperfusion in these patients, it was observed that the lipid peroxidation was accentuated in them as reflected in their statistically significant increase inMDAand a decrease in serumSOD.

Oxidative Stress And Enzymatic Antioxidants In Acute Myocardial Infarction

ABSTRACT
Myocardial infarction (MI) is the single largest killer disease in the world. Oxidative stress is one  of the hallmarks in patients with acute myocardial infarction as observed in recent years.The objective of the study was to evaluate oxidative stress and antioxidant enzyme activity in patients of acute MI.The present study comprised of 50 clinically diagnosed and ECG confirmed cases of acute MI and 50 healthy controls. Oxidative stress marker malondialdehyde (MDA) and enzymatic antioxidants like
superoxide dismutase (SOD), catalase and glutathione reductasewere estimated in the cases and controls.Statistical analysis  as carried out by using students’t’ test (unpaired). Correlation analysiswas done by using SPSS software version 16.The MDA level was significantly increased (P< 0.0001) in the cases (11.5 ± 0.72 nmol/ml) as compared to controls (6.3 ± 0.92 nmol/ml). The activity of SOD, glutathione reductase and catalasewere significantly low (P< 0.0001) in acuteMI (450.0 ± 116.76 IU/g Hb, 3.1 ± 0.79
IU/g of Hb and 2.6 ± 0.53 IU/g of Hb respectively) as compared to controls (940.4 ± 93.9 IU/g of Hb, 9 ± 1.02  IU/g of Hb and 7.3 ± 0.70 IU/g of Hb respectively). A significant negative correlation was detected between malondialdehyde and catalase activity in acute MI (r= – 0.339; p<0.02).The study indicates increased oxidative stress and decreased antioxidant defence in patients of acutemyocardial infarction. Antioxidant supplements are compounds obtained either by extraction from natural foods (dietary ntioxidants) or by chemical synthesis  (synthetic antioxidants). Many epidemiological data suggest that synthetic antioxidant supplementation may have a beneficial effect inmany chronic diseases. So novel t h e ra p e u t i c approaches l i ke ant i ox i d a nt supplementation may be useful in the management of persisting oxidative stress in acuteMI.