Antimicrobial resistance is an emerging problem in health care settings. One of the common mechanisms of antimicrobial resistance is production of Extended- Spectrum Beta-Lactamase (ESBL) by Gram Negative Bacilli. Knowledge about the prevalence of ESBL producing strains in the local area must be obtained for proper therapeutic interventions in infections caused by them.The purpose of the study was to detect the prevalence of Extended-Spectrum Beta-Lactamase producing Enterobacteriaceae isolates from various infections in a Tertiary Care Hospital, which guides in therapeutic management of such infections.