Context: Application of mupirocin to the anterior nares of patients colonized with Staphylococci eliminates the carriage of these organisms and is important in preventing the spread and development of Staphylococcal infection. There are several reports of Staphylococcal resistance to mupirocin.

Aim: This study was carried out primarily to determine the prevalence of low level and high-level mupirocin resistance in Staphylococcus aureus and coagulase-negative Staphylococci (CONS) and secondarily to compare its association with methicillin resistance.

Materials and Methods: Study sample included nasal swabs from a total of 206 subjects, which consisted of 104 medical students and 102 health care workers. Resistance to mupirocin was detected by disc diffusion method of Kirby Bauer using 5 μg and 200 μg mupirocin discs. Statistical analysis was done by Z test.

Results: In the present study, low-level mupirocin resistance was seen in 1 (2.23%) of methicillin-resistant Staphylococcus aureus (MRSA) and 3 (4.92%) of methicillinresistant CONS (MRCONS). High-level mupirocin resistance was seen in 2 (4.45%) of MRSA, 1 (1.58%) of methicillin- sensitive CONS, and 10 (16.39%) MRCONS isolates.

Conclusions: Overall mupirocin resistance in Staphylococcal isolates is still low. It is more prevalent in MRCONS species.