Healthcare-Associated Infections (HAI) are a major cause of morbidity and mortality. Standard Infection control practices are very important in reducing the incidence of HAI. The aim of this study was to assess infection control measures and its impact in the Medical Intensive Care Unit (MICU) of a tertiary care hospital. The Infection control practices studied include Hand hygiene practices, use of Personal Protective Equipment, Environmental Cleaning and Disinfection, Waste segregation and disposal, Aseptic precaution during Intravenous Cannulation, Urinary Catheterization and Ventilator care. Strict implementation of the Infection control practices resulted in a decrease in the HAI rate. So it is mandatory to have an effective infection control programme in every hospital.